AAT Bioquest

Does ethidium bromide bind ssDNA?

Posted November 10, 2021


Yes, ethidium bromide binds to single-stranded DNA. However, the binding is not as strong as its binding to double-stranded DNA resulting in lesser distortions, which makes detection less efficient. When using single-stranded DNA, it requires around 10 times more nucleic acid for equivalent detection.

Additional resources

The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures

Helixyte™ Green Fluorimetric ssDNA Quantitation Kit *Optimized for Microplate Readers*