Name: Helixyte™ Green Fluorimetric ssDNA Quantitation Kit *Optimized for Microplate Readers*
Brand: AAT Bioquest
SKU: 17623
Price: 213 USD
Availability: InStock

Helixyte™ Green Fluorimetric ssDNA Quantitation Kit

Optimized for Microplate Readers

Helixyte™ Green Fluorimetric ssDNA Quantitation Kit is designed to measure single-stranded DNA in an easy and accurate format. The kit has all the essential reagents, including Helixyte™ Green ssDNA reagent, dilution buffer, and prediluted DNA standards. Helixyte™ Green ssDNA reagent is a sensitive fluorescent nucleic acid probe for quantifying oligonucleotides and single-stranded DNA (ssDNA) in solution. It enables researchers to quantify as little as 100 pg/mL oligonucleotide or ssDNA. This sensitivity exceeds that achieved with absorbance methods by more than 10,000-fold. With this kit, as little as 1 ng/mL oligonucleotide or ssDNA might be detected. A few ssDNAs were quantified with this kit, including M13 and ϕX174 viral DNA and denatured calf thymus DNA that had similar sensitivity. Their detection limits were not significantly interfered by the common contaminants in nucleic acid preparations, including salts, urea, ethanol, chloroform, detergents, proteins, ATP, nucleotides and short oligonucleotides of six bases. However, double-stranded DNA (dsDNA) and RNA do interfere with the assay as Helixyte™ Green ssDNA reagent binds to dsDNA and RNA to generate additional fluorescence signals. Helixyte™ Green Fluorimetric ssDNA Quantitation Kit is optimized for quantifying ssDNA with a fluorescence microplate reader.

The ssDNA dose response was measured with Amplite® Fluorimetric ssDNA Quantitation Kit in a 96-well solid black plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17623	200 Tests	Price

References

View all 24 references: Citation Explorer

An OliGreen-responsive fluorescence sensor for sensitive detection of organophosphorus pesticide based on its specific selectivity towards T-Hg2+-T DNA structure.

Authors:

Zhou, Xiaoyuan and Wang, Chenchen and Wu, Lina and Wei, Wei and Liu, Songqin

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119155

A novel detection of radon based on its decay product inducing conformational changes of an aptamer probe.

Authors:

Long, Minzhi and Deng, Han and Tian, Gang and Song, Chunli and Liu, Hongwen and Shen, Yi and Lv, Changyin

Journal:

Analytica chimica acta (2016): 202-7

Studies of DNA Aptamer OliGreen and PicoGreen Fluorescence Interactions in Buffer and Serum.

Authors:

Bruno, John G and Sivils, Jeffrey C

Journal:

Journal of fluorescence (2016): 1479-87

Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.

Authors:

Morales, Magali and Attai, Hedieh and Troy, Kimberly and Bermudes, David

Journal:

Plasmid (2015): 7-16

Probing the inherent stability of siRNA immobilized on nanoparticle constructs.

Authors:

Barnaby, Stacey N and Lee, Andrew and Mirkin, Chad A

Journal:

Proceedings of the National Academy of Sciences of the United States of America (2014): 9739-44

Spectral properties

Excitation (nm)	498
Emission (nm)	519

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 11, 2025

Component A: Helixyte™ Green ssDNA	1 vial (200 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: ssDNA Standard	30 µL (100 µg/mL)