Name: Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *Optimized for Microplate Readers*
Brand: AAT Bioquest
SKU: 17650
Price: 232 USD
Availability: InStock

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit

Optimized for Microplate Readers

Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. It is specific for dsDNA in the presence of equimolar amounts of RNA. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. It can also be used with a bench top fluorometer or a hand-held fluorescence meter (e.g., Qubit fluorometer).

Comparison of dsDNA dose response using the Helixyte Green™ (blue ) with Invitrogen<sup>TM</sup> Quant-iT™ PicoGreen® dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17650	200 Tests	Price

References

View all 31 references: Citation Explorer

Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay

Authors:

Peterson EJ, Kireev D, Moon AF, Midon M, Janzen WP, Pingoud A, Pedersen LC, Singleton SF.

Journal:

J Biomol Screen (2013): 247

Validation of a PicoGreen-based DNA quantification integrated in an RNA extraction method for two-dimensional and three-dimensional cell cultures

Authors:

Chen Y, Sonnaert M, Roberts SJ, Luyten FP, Schrooten J.

Journal:

Tissue Eng Part C Methods (2012): 444

Characterization of PicoGreen interaction with dsDNA and the origin of its fluorescence enhancement upon binding

Authors:

Dragan AI, Casas-Finet JR, Bishop ES, Strouse RJ, Schenerman MA, Geddes CD.

Journal:

Biophys J (2010): 3010

Comparison of SYBR Green I-, PicoGreen-, and [3H]-hypoxanthine-based assays for in vitro antimalarial screening of plants from Nigerian ethnomedicine

Authors:

Abiodun OO, Gbotosho GO, Ajaiyeoba EO, Happi CT, Hofer S, Wittlin S, Sowunmi A, Brun R, Oduola AM.

Journal:

Parasitol Res (2010): 933

Metal-enhanced PicoGreen fluorescence: application to fast and ultra-sensitive pg/ml DNA quantitation

Authors:

Dragan AI, Bishop ES, Casas-Finet JR, Strouse RJ, Schenerman MA, Geddes CD.

Journal:

J Immunol Methods (2010): 95

Spectral properties

Excitation (nm)	502
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
UNSPSC	41116134

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on September 25, 2025

Component A: Helixyte Green™	1 vial (100 µL, 200X in DMSO)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Calf thymus DNA Standard	1 vial (200 µL, 100 µg/mL)