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Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Posted December 9, 2021
Cell Viability
Cell Cytotoxicity
Cellular Processes
Apoptosis and Necrosis
Flow Cytometry Reagents
Answer
Yes, it does.
Annexin-Phosphatidylserine binding
is lost below pH 5.2 and with prolonged incubation over a temperature of 42°C.
Additional resources
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Annexin-V Staining
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Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
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