logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Can 7-AAD stain live cells?
Posted December 10, 2021
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and Necrosis
Answer

No, 7-AAD (7-amino-actinomycin D) cannot stain live cells.

7-AAD is a fluorescent intercalating dye with a strong affinity for DNA. However it is membrane-impermeant and cannot pass through intact cell membranes, preventing it from being able to stain live cells. It can only stain necrotic and late apoptotic cells with compromised membrane integrity.

Additional resources
Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image CytometryCell Cycle Assays Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors*

Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?Does 7-AAD stain necrotic cells?What kind of cells does BrdU (Bromodeoxyuridine) stain?What is the difference between cell viability and cell proliferation?Is BrdU (Bromodeoxyuridine) toxic?