Can 7-AAD stain live cells?
Posted December 10, 2021
No, 7-AAD (7-amino-actinomycin D) cannot stain live cells.
7-AAD is a fluorescent intercalating dye with a strong affinity for DNA. However it is membrane-impermeant and cannot pass through intact cell membranes, preventing it from being able to stain live cells. It can only stain necrotic and late apoptotic cells with compromised membrane integrity.