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Can BrdU (Bromodeoxyuridine) be used in vitro?

Posted September 10, 2021


Answer

Yes, BrdU (Bromodeoxyuridine) can be in vitro as well as in vivo in cell proliferation studies. There are several different protocols for BrdU labeling in vitro. In general, primary cell cultures or cell lines can be incubated with BrdU at a final concentration of 10 μM (diluted in cell culture medium). As with all procedures, it is important that the protocol be optimized for your specific assay system before proceeding. 

Additional resources

An in vitro and in vivo study of a BrdU-sensitive fragile site in the Chinese hamster

Apoptosis and Necrosis

Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Green Fluorescence*