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How are inflammasomes detected?

Posted October 11, 2021


Answer

Inflammasomes are detected by immunoblotting with antibodies that are raised against the main inflammasome components. Priming of the cells prior to detection is an important step for detecting some of the inflammasome components. Inflammasome priming accomplishes two functions – it specifically affects NLRP3 at the transcriptional level and also serves to trigger posttranslational modifications of inflammasome components that allow for oligomerization.

Additional resources

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Caspases

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*