AAT Bioquest

How do I optimize my cell line for transfection?

Posted February 19, 2020


There are multiple ways to improve your transfection results with a particular cell line and reagent.

We suggest the following:


  • Determine cell line transfection resistance


  • Older reagent formulations have longer protocols, but more documentation of cell line compatibility


  • Suspended cells often require higher densities than adherent cultures

  • Scientific support and assistance from reagent manufacturer


Additional resources

Transfectamine™ 5000 Transfection Reagent