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How do I optimize my cell line for transfection?
Posted February 19, 2020

Answer

There are multiple ways to improve your transfection results with a particular cell line and reagent.

We suggest the following:

 

  • Determine cell line transfection resistance

 

  • Older reagent formulations have longer protocols, but more documentation of cell line compatibility

 

  • Suspended cells often require higher densities than adherent cultures

  • Scientific support and assistance from reagent manufacturer