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How do I optimize my cell line for transfection?
Posted February 19, 2020
Transfectionadherent cellscell linessuspended cells
Answer
There are multiple ways to improve your transfection results with a particular cell line and reagent.
We suggest the following:
 
  • Determine cell line transfection resistance
 
  • Older reagent formulations have longer protocols, but more documentation of cell line compatibility
 
  • Suspended cells often require higher densities than adherent cultures
  • Scientific support and assistance from reagent manufacturer
 
Additional resources
Transfectamine™ 5000 Transfection Reagent

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