Your optimal protocol will depend on multiple factors including your chosen cell line, but we do have a suggested protocol for our transfection reagent.
5. Analyze transfection efficiency with appropriate method
Maximum expression levels can be discerned 72-96 hours post-transfection
If using Flow Cytometry, Laser-Scanning Molecular Imaging, Microscopy, or Western Blot Analysis, a fluorescent label is necessary or strongly recommended