What level of DNA purity is best for transfection?
Posted February 19, 2020
Although there is a range for DNA purity and concentration, there are several factors that may impact a particular procedure, including the selected transfection reagent.
- Compare DNA/Reagent concentrations for best performance
- Test different incubation periods of DNA and reagent before cell mixing
- Endotoxin levels should be kept as low as possible
- A260/A280of 1.7-1.9 are ideal purity levels for most procedures-try in this range first and adapt from there