Although there is a range for DNA purity and concentration, there are several factors that may impact a particular procedure, including the selected transfection reagent.

We suggest:

• Compare DNA/Reagent concentrations for best performance

• Test different incubation periods of DNA and reagent before cell mixing

• Endotoxin levels should be kept as low as possible

• A₂₆₀/A₂₈₀of 1.7-1.9 are ideal purity levels for most procedures-try in this range first and adapt from there