AAT Bioquest

What level of DNA purity is best for transfection?

Posted February 19, 2020


Although there is a range for DNA purity and concentration, there are several factors that may impact a particular procedure, including the selected transfection reagent.

We suggest:


  • Test different incubation periods of DNA and reagent before cell mixing


  • Endotoxin levels should be kept as low as possible


  • A260/A280of 1.7-1.9 are ideal purity levels for most procedures-try in this range first and adapt from there


Additional resources

Transfectamine™ 5000 Transfection Reagent