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How do I prepare my cells for transfection?
Posted February 19, 2020

Answer

If you are developing an in-house procedure or making use of a new transfection reagent, we suggest these initial preparation steps:

 

 

  • 70-90% confluency recommended for most cell lines

 

  • Minimize required reagent to keep toxicity low

 

  • Prepare cell culture 24 hours prior to procedure for optimal replication state