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How do I prepare my cells for transfection?
Posted February 19, 2020
Answer
If you are developing an in-house procedure or making use of a new transfection reagent, we suggest these initial preparation steps:
- An initial cell viability assay to ascertain cell health
- 70-90% confluency recommended for most cell lines
- Minimize required reagent to keep toxicity low
- Prepare cell culture 24 hours prior to procedure for optimal replication state
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