AAT Bioquest

How do I prepare my cells for transfection?

Posted February 19, 2020


If you are developing an in-house procedure or making use of a new transfection reagent, we suggest these initial preparation steps:



  • 70-90% confluency recommended for most cell lines


  • Minimize required reagent to keep toxicity low


  • Prepare cell culture 24 hours prior to procedure for optimal replication state
Additional resources

Transfectamine™ 5000 Transfection Reagent