AAT Bioquest

How do I determine transfection efficiency using a flow cytometer?

Posted February 19, 2020


Using flow cytometry, determining the success of a particular transfection procedure is quite simple:


  • Use an untransfected control and subtract the baseline intensity data from your experimental results
  • Signal intensity graphs are a simple visual aid to compare the response of the cell line to different transfection reagents

Manual analysis is likely unnecessary; most instruments have software with FACS analysis programs for quick results.

Additional resources

Flow Cytometry Application Notes

Flow Cytometer Assay Kits

Transfectamine™ 5000 Transfection Reagent