How do I determine transfection efficiency using a flow cytometer?
Posted February 19, 2020
Using flow cytometry, determining the success of a particular transfection procedure is quite simple:
- Use an untransfected control and subtract the baseline intensity data from your experimental results
- Signal intensity graphs are a simple visual aid to compare the response of the cell line to different transfection reagents
Manual analysis is likely unnecessary; most instruments have software with FACS analysis programs for quick results.