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How do I determine transfection efficiency using a flow cytometer?
Posted February 19, 2020

Answer

Using flow cytometry, determining the success of a particular transfection procedure is quite simple:

 

  • Use an untransfected control and subtract the baseline intensity data from your experimental results
  • Signal intensity graphs are a simple visual aid to compare the response of the cell line to different transfection reagents

Manual analysis is likely unnecessary; most instruments have software with FACS analysis programs for quick results.