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How do I remove protein tags after purification?
Posted June 22, 2020
Answer
With the attachment of protein tags, the native function of protein may be abolished or compromised. Therefore, after purification, tags are sometimes removed by specific proteases (e.g. TEV protease and Thrombin), whose target sites are engineered as part of the expressed protein.
Additional resources
Helixyte™ Green *10,000X Aqueous PCR Solution*
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*Perdew, G. H., Heuvel, J. P. V., & Peters, J. M. (2008). Regulation of gene expression. Springer Science & Business Media.Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.