What is the difference between BCA and Micro BCA assay? | AAT Bioquest

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What is the difference between BCA and Micro BCA assay?

Posted April 24, 2020

Antibodies and Proteomics Affinity Chromatography BCA assay Bradford assay Chromatographic techniques Extraction Protein Protein Purification Protein concentration

Answer

While both assays use BCA (bicinchoninic acid) to measure protein concentration of dilute protein samples, the Micro BCA assay is designed for testing proteins in lower sample concentrations of 0.5-20 ug/ml, while the standard BCA assay works in the range of 20-2000 ug/ml.

Additional resources

AmpliteTM Fluorimetric Protein Quantitation Kit *Orange Fluorescence*Huang T., Long M., & Huo B. (2010). Competitive Binding to Cuprous Ions of Protein and BCA in the Bicinchoninic Acid Protein Assay. Open Biomedical Engineering Journal, 14, 271–278. doi:10.2174/1874120701004010271 Protein Concentration Calculator Bradford Protein Assay Calculator

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