logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
How does CRISPR-Cas9 edit genes?
Posted June 22, 2020
DNADNA and RNADNA sequencingEnzymesbacteria
Answer

CRISPR-Cas9 cuts genes by creating a double-strand breaks (DSB) in the DNA. Then innate DNA repair mechanisms are automatically triggered within the cell, which can either leave the sequences deleted or insert specific sequences of interest, resulting precise gene editing.

Additional resources
Helixyte™ Green *10,000X Aqueous PCR Solution* 6-ROX glycine *25 uM fluorescence reference solution for PCR reactions* Doudna, J. A., & Charpentier, E. (2014). The new frontier of genome engineering with CRISPR-Cas9. Science, 346(6213), 1258096. Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome engineering using the CRISPR-Cas9 system. Nature protocols, 8(11), 2281.

What is difference between CRISPR interference and CRISPR activation?How is Cas prevented from cutting its own CRISPR locus?How is CRISPR-Cas9 used in gene knockout and knock-in?What are the three stages in the CRISPR-Cas defense system?What are the two main types of DNA repair mechanisms employed in CRISPR-Cas9?