AAT Bioquest

How long does next-generation sequencing (NGS) take?

Posted May 9, 2020


The length of time that next-generation sequencing takes depends on the platform and size of the genome being sequenced, so it can take anywhere from 3 hours to around a day or more. For example, sequencing with the Ion PGM can take around 3 hours, while Illumina’s NextSeq 500 and MiSeq may take anywhere between 15-26 hours and 5-55 hours, respectively.

Additional resources

Alekseyev, Y. O., Fazeli, R., Yang, S., Basran, R., Maher, T., Miller, N. S., & Remick, D. (2018). A next-generation sequencing primer—how does it work and what can it do?. Academic pathology, 5, 2374289518766521.