How to lyse cells?

Can you provide the recommended cell volume and lysis buffer volume. Is sonication required?

Overview:

• Cell Volume: 1∼2 X 10⁶ cells
• Lysis Buffer Volume: 100 µL
• No sonication required

Protocol: Cell Lysis

1. Remove medium and wash cells once with HHBS buffer (Cat#20011).
2. For each assay, pellet 1∼2 X 10⁶ cells in a micro-centrifuge tube.
3. Add 100 µL lysis buffer to cell pellet.
4. Incubate for 15 minutes at 37°C.
5. Centrifuge for 10 minutes at 5000 RPM to precipitate cell pellet.
6. Decant the supernatant to a fresh tube and discard the cell pellet (cell number/sample = 2.5∼5 X 10⁵).