AAT Bioquest

How to lyse cells?

Posted October 21, 2019

Can you provide the recommended cell volume and lysis buffer volume. Is sonication required?



  • Cell Volume: 1∼2 X 106 cells
  • Lysis Buffer Volume: 100 µL
  • No sonication required

Protocol: Cell Lysis

  1. Remove medium and wash cells once with HHBS buffer (Cat#20011).
  2. For each assay, pellet 1∼2 X 106 cells in a micro-centrifuge tube.
  3. Add 100 µL lysis buffer to cell pellet.
  4. Incubate for 15 minutes at 37°C.
  5. Centrifuge for 10 minutes at 5000 RPM to precipitate cell pellet.
  6. Decant the supernatant to a fresh tube and discard the cell pellet (cell number/sample = 2.5∼5 X 105).
Additional resources

HHBS [Hanks' Buffer with 20 mM Hepes]