AAT Bioquest

How to prepare cell lysate for NADP/NADPH ratio assay?

Posted October 22, 2019


Protocol: NADP/NADPH Ratio Assay, Cell Lysate, HL-60 cells

  1. 1 mL of HL-60 cells (1∼2x106 cells/mL)
  2. Centrifuge to remove medium
  3. Add 200 µL of lysis buffer to the cell pellet
  4. Incubate for 15 minutes at 37°C
  5. Centrifuge for 5 minutes to remove cell pellet
  6. The supernatant can be used directly as your sample
Additional resources

Amplite™ Colorimetric NADP/NADPH Ratio Assay Kit

Amplite™ Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*