AAT Bioquest

Is Cy3 a fluorophore?

Posted July 1, 2021


Yes, Cy3 is an orange fluorophore. It has an absorption wavelength that peaks around 548-552 nm and an emission maximum around 562-570 nm.

Cy3 is commonly used in a wide range of applications such as fluorescence microscopy, nucleic acid labeling, immunolabeling, and flow cytometry.

Additional resources

Membrane Interactions of Cy3 and Cy5 Fluorophores and Their Effects on Membrane-Protein Dynamics

Cyanine 3 monosuccinimidyl ester [equivalent to Cy3® NHS ester]