Is ethidium bromide used in RT PCR? | AAT Bioquest

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Is ethidium bromide used in RT PCR?

Posted November 30, 2021

Nucleus Physiological Probes DNA and RNA Quantitation Chemical Reagents

Answer

Ethidium bromide can be used in RT PCR. However, because of its highly carcinogenic and mutagenic nature, SYBR Green, which is a safe alternative, is generally used in RT PCR.

Additional resources

Ethidium monoazide does not inhibit RT-PCR amplification of nonviable avian influenza RNA Q4ever™ Green *1250X DMSO Solution*6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*

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