Ethidium bromide can be used in RT PCR. However, because of its highly carcinogenic and mutagenic nature, SYBR Green, which is a safe alternative, is generally used in RT PCR.
Ethidium monoazide does not inhibit RT-PCR amplification of nonviable avian influenza RNA
Q4ever™ Green *1250X DMSO Solution*
6-ROXtra™ fluorescence reference solution *25 uM for PCR reactions*