Is qPCR efficiency under 90% acceptable?
Posted November 24, 2020
To put it bluntly, No!
qPCR efficiency under 90% is not acceptable. The efficiency should be between 90 to 110%. If you are experiencing less than 90% efficiency you may have:
- Poorly designed PCR primers
- PCR inhibitors in your samples
- Inconsistent pipetting techniques leading to inaccurate sample and reagent pipetting
Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*