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Is qPCR efficiency under 90% acceptable?

Posted November 24, 2020


Answer

To put it bluntly, No!

qPCR efficiency under 90% is not acceptable. The efficiency should be between 90 to 110%. If you are experiencing less than 90% efficiency you may have:

  • Poorly designed PCR primers
  • PCR inhibitors in your samples
  • Inconsistent pipetting techniques leading to inaccurate sample and reagent pipetting
Additional resources

Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*

Polymerase Chain Reaction (PCR)