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What are the differences between next-generation sequencing (NGS) and classical Sanger sequencing?
Posted July 22, 2020
Answer
The main differences between NGS and Sanger sequencing are sequencing volume, sequencing length, and cost.
- Sequencing volume: Sanger sequencing can only sequence one single fragment at a time, whereas NGS can read millions of DNA pieces in the flow cell simultaneously per run.
- Sequencing length: NGS methods usually read fragments 100-200 bases long, while Sanger sequencing can read as long as 700-1000 bases in a single sequence.
- Cost: Because of the high-throughput feature of NGS, it is much more cost-effective than Sanger sequencing when sequencing a large amount of DNA.
Additional resources
Helixyte™ Green *10,000X Aqueous PCR Solution*
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions* Krishna, B. M., Khan, M. A., & Khan, S. T. (2019). Next-Generation Sequencing (NGS) Platforms: An Exciting Era of Genome Sequence Analysis. In Microbial Genomics in Sustainable Agroecosystems (pp. 89-109). Springer, Singapore. Ju, J., Kim, D. H., Bi, L., Meng, Q., Bai, X., Li, Z., ... & Edwards, J. R. (2006). Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators. Proceedings of the National Academy of Sciences, 103(52), 19635-19640.