AAT Bioquest

What are the essential features of a plasmid cloning vector?

Posted June 22, 2020


To become a successful plasmid cloning vector, it must be a small DNA molecule that can be self-replicating inside host cells, which generally has several essential features as follows:

  • Origin of replication: It is the site where replication is initiated.
  • Marker genes for selection and/or screening: These genes allow to screen out cells that lack specific genes, e.g. antibiotic resistance genes, and also are used to test difference in phenotype between cells.
  • Unique and multiple restriction sites: The restriction sites for various restriction endonucleases allow insertion of foreign DNA to specific sites on plasmid.
  • Promoters for gene expression: It is an essential component of expression vectors, which drives the transcription of the vector’s transgene and helps in getting the cloned product expressed.
Additional resources

Helixyte™ Green *10,000X Aqueous PCR Solution*

6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*

Perdew, G. H., Heuvel, J. P. V., & Peters, J. M. (2008). Regulation of gene expression. Springer Science & Business Media.

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.