What are the limitations of Taq polymerase?
Posted June 22, 2020
- Low specificity: Taq DNA polymerase has a lower specificity than the normal ones. Mismatches nucleotides could be added to the sequence by Taq polymerase.
- Low fidelity: Taq polymerase does not have 3’ to 5’ exonuclease proofreading activity, therefore mismatches nucleotides could not be corrected.
- Bivalent cation requirement: Taq polymerase requires cofactors, such as Mg2+ ion, to work properly.
Chen, S. C., Halliday, C. L., & Meyer, W. (2002). A review of nucleic acid-based diagnostic tests for systemic mycoses with an emphasis on polymerase chain reaction-based assays. Medical mycology, 40(4), 333-357.