What are the three stages of a PRC process?
Posted June 22, 2020
PCR process can be divided into three stages based on reaction progress:
- Exponential amplification: The amount of product is doubled at every cycle when DNA polymerase is fully active, and reagents are abundant.
- Leveling off stage: Reaction rate slows as the DNA polymerase loses activity and as consumption of reagents.
- Plateau: No more product accumulates due to exhaustion of reagents and enzyme.
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