Calculator
What are the three stages of a PRC process?
Posted June 22, 2020
Answer
PCR process can be divided into three stages based on reaction progress:
- Exponential amplification: The amount of product is doubled at every cycle when DNA polymerase is fully active, and reagents are abundant.
- Leveling off stage: Reaction rate slows as the DNA polymerase loses activity and as consumption of reagents.
- Plateau: No more product accumulates due to exhaustion of reagents and enzyme.
Additional resources
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*