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What are the three stages of a PRC process?
Posted June 22, 2020
DNADNA and RNA QuantitationEnzymesPCRPCR stepsPolymerase Chain Reaction (PCR)PolymerasesReal-Time PCR (qPCR)
Answer
PCR process can be divided into three stages based on reaction progress:
- Exponential amplification: The amount of product is doubled at every cycle when DNA polymerase is fully active, and reagents are abundant.
- Leveling off stage: Reaction rate slows as the DNA polymerase loses activity and as consumption of reagents.
- Plateau: No more product accumulates due to exhaustion of reagents and enzyme.
Additional resources
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*Chen, S. C., Halliday, C. L., & Meyer, W. (2002). A review of nucleic acid-based diagnostic tests for systemic mycoses with an emphasis on polymerase chain reaction-based assays. Medical mycology, 40(4), 333-357.Chuang, L. Y., Cheng, Y. H., & Yang, C. H. (2013). Specific primer design for the polymerase chain reaction. Biotechnology letters, 35(10), 1541-1549.