AAT Bioquest

What is a cofactor?

Posted July 22, 2020


A cofactor is a non-protein molecule that is required by an enzyme to execute its catalytic activity, which is considered a “helper molecule” that assists in biochemical transformations. Cofactors can be divided into two main types: inorganic cofactors such as metal ions and iron-sulfur clusters, and organic cofactors, also known as coenzymes, such as flavin and heme.

Additional resources

Amplite™ Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Amplite™ Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*

Lodish, H., Berk, A., Kaiser, C. A., Krieger, M., Scott, M. P., Bretscher, A., ... & Matsudaira, P. (2008). Molecular cell biology. Macmillan.

Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.