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AAT Bioquest

What is lipid peroxidation?

Posted June 13, 2019


How do you detect and measure it?

Answer

Lipid peroxidation is the oxidation of lipids, most often affecting polyunsaturated fatty acids. Peroxidation of unsaturated lipids alters cell membrane integrity as well as cell apoptosis and cell signaling pathways. Lipid peroxidation also plays a role in aging, atherosclerosis and free radical mediated cell damage.

Our Cell Meter™ Colorimetric Lipid Peroxidation (MDA) Quantitation Kit offers the most convenient and rapid method for measuring lipid peroxidation without the TBARS heating steps. It utilizes our MDA Blue™ sensor to detect malondialdehyde, a commonly used biomarker for lipid peroxidation. Upon reacting with MDA, MDA Blue™ will generate a blue colored product which is measured at an absorbance wavelength of 695 nm. This assay is specific to MDA with little interference from other aldehydes.

Additional resources

Protocol: Cell Meter™ Colorimetric Lipid Peroxidation (MDA) Quantitation Kit

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit