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What is primary cell culture?

Posted July 22, 2020


Cells that are directly isolated from tissues using enzymatic or mechanical methods are referred to as primary cells. Primary cell culture is the process that cultures primary cells, which proliferate under appropriate conditions until they occupy desirable confluence. After that, the proliferated cells undergo sub-culturing, when they are transferred to a new culture vessel with fresh growth medium to provide more room for continued growth. After the first subculture, the primary cell culture becomes a cell line.

Additional resources

Cell Cycle and Proliferation

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Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.

Yao, T., & Asayama, Y. (2017). Animal?cell culture media: History, characteristics, and current issues. Reproductive medicine and biology, 16(2), 99-117.

Butler, M. (2004). Animal cell culture and technology. Taylor & Francis.

Cell Sample Preparation