Polymerase chain reaction (PCR) is a quick and easy method used to amplify DNA sequences in vitro, which is based on the nucleic acid enzymatic replication principle.
The nucleic acid enzymatic replication principle consists of a four step process that entails:
DNA denaturation
Primer annealing to target sequence
Target sequence extension
End of the cycle, after which the cycle is repeated to amplify DNA to reach the target number of DNA replicates