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What is the best fixation for fluorescence microscopy of GFP tagged proteins?
Posted April 17, 2020
FixationPBSfixed cellsfluorescence microscope
Answer

For fluorescence microscopy of GFP tagged proteins, the best method for fixation is to use fresh 4% PFA (paraformaldehyde) in PBS, buffered to 7.4 pH. The cells should be fixed for 15-30 min in room temperature and then washed well with PBS before moving on to blocking and permeabilization.

Additional resources
ReadiUse™ 4% formaldehyde fixation solutionPBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe

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