logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
What is the difference between 7-AAD and Propidium Iodide?
Posted December 23, 2021
DNA and RNA QuantitationNucleusBioconjugationPhysiological Probes
Answer

7-AAD andPropidium Iodide are both fluorescent, intercalating agents that can be used to stain cells and nucleic acids. The major difference between the two is that 7-AAD binds to double-stranded DNA by intercalating between base pairs in G-C-rich regions of DNA, while Propidium Iodide binds to DNA by intercalating between the bases with little or no sequence preference.

Additional resources
Comparison between propidium iodide and 7-amino-actinomycin-D for viability assessment during flow cytometric analyses of the human sperm acrosome DNA and RNA QuantitationDAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*

Which viruses use reverse transcriptase?What are the building blocks of nucleic acids?What are the steps of reverse transcription?What are the examples of eukaryotic cells?What does Giemsa bind to?