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What is the difference between 7-AAD and Propidium Iodide?
Posted December 23, 2021
Answer
7-AAD and Propidium Iodide are both fluorescent, intercalating agents that can be used to stain cells and nucleic acids. The major difference between the two is that 7-AAD binds to double-stranded DNA by intercalating between base pairs in G-C-rich regions of DNA, while Propidium Iodide binds to DNA by intercalating between the bases with little or no sequence preference.
Additional resources
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*