What is the difference between assay and purity?
Posted January 8, 2021
Answer
Assay and purity are two types of measurements used to determine the components of a sample. serve different purposes.
Assay
- Assay experimentally determines the presence, amount, or functional activity of a target entity (the analyte)
- It aims to measure the analyte or main target component present in the sample.
- The measurement may be quantitative, semi-quantitative, qualitative or functional.
- Endpoint assays and kinetic assays are the two types of assays used, based on type and number of measurements taken. Endpoint assays measure signals after a specific incubation period. Kinetic assays take multiple measurements over fixed intervals.
- Assays may also be single target or multiplex. Single target essays measure one component at a time in a sample. Multiplex essays measure several components simultaneously during the same essay.
Purity
- Purity is an analysis of the impurities present in a sample.
- It aims to accurately reflect the purity characteristics of the sample.
- It is a quantitative measurement and is indicated as a percentage.
- Chromatography is the primary analytical method used to determine the purity of a sample. Both, liquid chromatography and gas chromatography are equally capable of measuring the purity of a sample.
Additional resources
Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit