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AAT Bioquest

What is the difference between loading dye and ethidium bromide?

Posted December 1, 2021


Answer

Loading dye is used for making the DNA markers. It is added to an electrophoresis sample to give it color and density. 

Ethidium bromide is used to stain DNA. It is a tracking dye used to visualize DNA in agarose gel electrophoresis experiments.

Additional resources

Ethidium bromide as a cooperative effector of a DNA structure

TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*

Real-Time PCR (qPCR)