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What is the difference between loading dye and ethidium bromide?
Posted December 1, 2021
Nucleus
Physiological Probes
DNA and RNA Quantitation
Answer
Loading dye is used for making the DNA markers. It is added to an electrophoresis sample to give it color and density.
Ethidium bromide is used to stain DNA. It is a tracking dye used to visualize DNA in agarose
gel electrophoresis
experiments.
Additional resources
Ethidium bromide as a cooperative effector of a DNA structure
TAQuest™ FAST qPCR Master Mix for TaqMan Probes *No ROX*
Real-Time PCR (qPCR)
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What is the difference between loading dye and ethidium bromide?
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