AAT Bioquest

What media should I use for my tissue culture?

Posted December 4, 2020


The type of media used in tissue culturing largely depends on the sample type and should be optimized accordingly. For plant tissue cultures the media generally contains a mixture of macronutrients, micronutrients, vitamins, hormones, nitrogen supplements, amino acids, growth regulators, organic supplements and solidifying agents. For mammalian cells the media contains a mixture of amino acids, vitamins, inorganic salts, glucose and other supplements. 

  • Common types of plant tissue culture media include Murashige and Skoog (MS) medium, Linsmaier and Skoog (LS) medium, Gamborg (B5) medium, Nitsch and Nitsch (NN) medium and White’s medium. 
  • Common types of mammalian cell culture media include Eagle minimal essential medium (MEM), Dulbecco’s modified Eagle medium (DMEM), RPMI 1640 and HamF10.
Additional resources

Influence of type of culture medium on characterization of Mycobacterium avium subsp. paratuberculosis subtypes

CellPaint™ TSP membrane stain