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AAT Bioquest

What's best for DNA quantification, gel electrophoresis or NanoDrop?

Posted January 8, 2021


Answer

NanoDrop Spectrophotometer is better for DNA quantification. Gel eletrophoresis is the better option to measure quality rather than quantity.

A NanoDrop Spectrophotometer is a very small spectrophotometer. It is capable of reading DNA concentration and purity accurately in as little as 1μl. It determines the concentration and purity of a particular substance in a liquid using the absorbance or transmission of light through the liquid.

Additional resources

Introduction and Perspectives of DNA Electrophoresis

Helixyte™ Green Fluorimetric dsDNA Quantitation Kit *High Sensitivity*

DNA and RNA