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Which method is better for measuring the amount of antibodies in a sample, ELISA or FACS?
Posted April 24, 2020
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)ELISAEnzymesPeptide and Oligonucleotide
Answer
FACS is a useful method for analyzing samples based on several parameters and is generally more sensitive. However, ELISA may also be sufficiently effective if simply wanting to measure the amount of antibodies in a sample.
Additional resources
Drouvalakis, K. A., Neeson, P. J., & Buchanan, R. R. (1999). Detection of anti-phosphatidylethanolamine antibodies using flow cytometry. Cytometry, 36(1), 46-51.Piechocki, M. P., Pilon, S. A., & Wei, W. Z. (2002). Quantitative measurement of anti-ErbB-2 antibody by flow cytometry and ELISA. Journal of immunological methods, 259(1-2), 33–42. doi:10.1016/s0022-1759(01)00487-2