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Why are tandem dyes not recommended for intracellular staining?
Posted September 23, 2021
BioconjugationPE and APC
Answer

Tandem dyes are not recommended for intracellular staining because their larger size makes it difficult for them to cross the cell membrane and reach the intracellular target proteins. This inability to cross the cell membrane makes them ineffective for intracellular staining.

Additional resources
Tandem dyes: Stability in cocktails and compensation considerationsReadiUse™ Preactivated PE-Cy5.5 Tandem

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