What are the 3 top guidelines for using PerCP conjugates and tandems?
Posted October 20, 2021
Phycobiliproteins—such as PE, APC, and PerCP—are intensely bright due to their high molar extinction coefficients and quantum yields; an enviable quality when imaging low-abundance targets. These fluorophores have extensive uses in flow cytometry and FACS, but there are some limits to their applications. Useful guidelines include:
- Phycobiliproteins photobleach rapidly, so they are not recommended for microscopy.
- Take experiment length into account. PerCP tandem structure may make it more photostable than PerCP alone. Choose the label best suited to time requirements.
- PerCP tandems are highly sensitive. A long-wavelength paired fluorophore may reduce background noise. Cy5 & Cy5.5 are common options, but select based on your experimental needs.