Why should isotype controls be used in immunofluorescence staining?
Posted August 21, 2019
Isotype controls are primary antibodies used as a negative control. They confirm that the binding of primary antibodies is specific to the target of interest and not a result of non-specific binding or other protein interactions. For example, if the target antibody is iFluor® 488-conjugated mouse IgG1, you should select a mouse IgG1 isotype control conjugated to iFluor® 488.
At AAT Bioquest, we provide Buccutite™ Rapid Antibody Labeling kits with wide variety of fluorophores, for 25 and 100 µg antibody labelling sizes. The kit includes preactivated fluorophores which facilitates conjugation very efficiently with the target antibody.