AAT Bioquest

Does DAPI stain all cells?

Posted December 10, 2022


DAPI is used to stain both fixed and live cells. It is a cell permeable dye with the ability to stain the DNA of eukaryotic and prokaryotic cells. The dye has a high affinity for the minor groove of the A-T DNA sequences. DAPI is preferred for fixed cell staining over live cell staining, since it takes higher concentrations to permeate the membrane.


Additional resources

DAPI as a useful stain for nuclear quantitation

DAPI - Blue Fluorescent DNA Stain

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

FluoroQuest™ Mounting Medium with DAPI