Does DAPI stain all cells?
Posted December 10, 2022
DAPI is used to stain both fixed and live cells. It is a cell permeable dye with the ability to stain the DNA of eukaryotic and prokaryotic cells. The dye has a high affinity for the minor groove of the A-T DNA sequences. DAPI is preferred for fixed cell staining over live cell staining, since it takes higher concentrations to permeate the membrane.