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Does DAPI stain all cells?
Posted December 10, 2022
Answer
DAPI is used to stain both fixed and live cells. It is a cell permeable dye with the ability to stain the DNA of eukaryotic and prokaryotic cells. The dye has a high affinity for the minor groove of the A-T DNA sequences. DAPI is preferred for fixed cell staining over live cell staining, since it takes higher concentrations to permeate the membrane.
Additional resources
DAPI as a useful stain for nuclear quantitation
DAPI - Blue Fluorescent DNA Stain
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*