How do I choose the right strategy for my gene editing experiment?

There are several factors to consider when choosing the right strategy for gene editing. The polycistronic transfer RNA-gRNA system offers several advantages over monocistronic strategies. These advantages include higher editing efficiency, efficient use of vector space, and the flexibility to utilize cell-type-specific promoters (such as Pol II promoters instead of Pol III). However, if the procedure involves cloning and testing gRNAs individually to confirm their effectiveness, a practical approach is to start by transfecting the cell line with a mixture of individually cloned gRNA plasmids. In the event of inadequate transfection or editing efficiency, a transition to a polycistronic approach can be considered later on.