What are the advantages of prime editing?

One advantage of prime editing is that it uses the cell's intrinsic DNA mismatch repair system to precisely incorporate changes into the genome, resulting in fewer unwanted or random by-products compared to other genome editing methods like CRISPR-Cas9. Prime editing also offers exceptional precision and flexibility in editing the genome. The use of single-stranded pegRNA allows for precise modifications across the DNA sequence, including all types of insertions, transitions and substitutions. Additionally, unlike traditional CRISPR-Cas9 editing, prime editing does not rely on the induction of double-strand breaks in the DNA or the use of donor DNA templates for repair. This reduces the risk of unintended mutations.