One advantage of prime editing is that it uses the cell's intrinsic DNA mismatch repair system to precisely incorporate changes into the genome, resulting in fewer unwanted or random by-products compared to other genome editing methods like CRISPR-Cas9. Prime editing also offers exceptional precision and flexibility in editing the genome. The use of single-stranded pegRNA allows for precise modifications across the DNA sequence, including all types of insertions, transitions and substitutions. Additionally, unlike traditional CRISPR-Cas9 editing, prime editing does not rely on the induction of double-strand breaks in the DNA or the use of donor DNA templates for repair. This reduces the risk of unintended mutations.