How does fluorescent detection work? | AAT Bioquest

Search by catalog number, product name, application...

Products

Services

Resources

Selection Guides

About

How does fluorescent detection work?

Posted May 2, 2024

Antibodies and Proteomics Bioconjugation Antibody and Protein Labeling Immunohistochemistry (IHC)

Answer

Fluorescent detection is based on using a fluorochrome conjugated antibody. On stimulation with a light of a shorter wavelength, the fluorochrome conjugated antibody emits light, enabling visualization.

Additional resources

Recent Advances in Fluorescent Probes for Cancer Biomarker Detection Immunohistochemistry (IHC) Stayright™ Purple HRP Staining Kit

What causes T cells to become exhausted?What are the characteristics of T cell exhaustion?What is the B cell receptor made up of?What's the role of BSA/Milk as blocking agents??What are types of T cells?