How does fluorescent detection work? | AAT Bioquest

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How does fluorescent detection work?

Posted May 2, 2024

Antibodies and Proteomics Bioconjugation Antibody and Protein Labeling Immunohistochemistry (IHC)

Answer

Fluorescent detection is based on using a fluorochrome conjugated antibody. On stimulation with a light of a shorter wavelength, the fluorochrome conjugated antibody emits light, enabling visualization.

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