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How is a DNA probe generated for Southern blotting?
Posted May 2, 2024

Answer

A DNA probe for Southern blotting is typically generated by designing a short, single-stranded DNA sequence that is complementary to the target DNA sequence. This complementary sequence ensures specific binding between the probe and the target DNA during hybridization. These probes can be made from DNA fragments obtained from cutting DNA with specific enzymes called restriction endonucleases or from short synthetic DNA sequences.