What are the differences between Southern blotting and other blotting techniques?

Question

Accepted Answer

Basis of differentiation

Southern Blotting

Northern blotting

Western blotting

Target molecule

DNA

RNA

Proteins

Sample preparation

DNA extraction and digestion

RNA extraction and separation

Protein extraction and denaturation

Membrane type

Nylon or nitrocellulose membranes

Nitrocellulose membranes

Probe type

Single-stranded DNA or RNA is used as a probe

cDNA or RNA probes are used with radioactive or non-radioactive labels

Antibodies (primary and secondary)

Blotting technique

Capillary transfer blotting technique

Electric transfer blotting technique

Detection method

Chemiluminescence, X-Ray film

CCD camera, Film or LED imaging system

Basis of differentiation	Southern Blotting	Northern blotting	Western blotting
Target molecule	DNA	RNA	Proteins
Sample preparation	DNA extraction and digestion	RNA extraction and separation	Protein extraction and denaturation
Membrane type	Nylon or nitrocellulose membranes	Nylon or nitrocellulose membranes	Nitrocellulose membranes
Probe type	Single-stranded DNA or RNA is used as a probe	cDNA or RNA probes are used with radioactive or non-radioactive labels	Antibodies (primary and secondary)
Blotting technique	Capillary transfer blotting technique	Capillary transfer blotting technique	Electric transfer blotting technique
Detection method	Chemiluminescence, X-Ray film	Chemiluminescence, X-Ray film	CCD camera, Film or LED imaging system