How is a mounting medium applied to a microscope slide?
Posted April 6, 2023
There is no single protocol for applying a mounting medium that’s ideal for all experiments. You will need to modify the general guideline below to better suit your specific requirements:
- Remove the mounting solution from the refrigerator and warm the bottle to room temperature. Make sure it is protected from light to minimize photobleaching.
- Remove excess liquid from your sample and apply a small drop of the mounting solution to the sample. If the sample is on a tissue culture dish or slide, take care to avoid air bubbles by carefully placing a coverslip on the drop. If the sample is on a coverslip, invert the coverslip on a clean glass slide.
- Remove any excess anti-fading agent.
- Prepare samples for imaging by first incubating the mounting solution for about two hours to overnight. Use a plastic sealant or nail polish to seal the coverslip to the slide for long-term storage. Store mounted slides in the dark at 4 °C for optimum sample longevity. This will allow fluorescence imaging to remain stable for several weeks. Samples are ready to be imaged immediately after mounting.