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AAT Bioquest

What are the advantages of column chromatography?

Posted August 23, 2023


Answer

One advantage is that many different kinds of mixtures can be separated using this technique. For example, small molecules, such as salts, can be separated from larger molecules like protein. It also allows for the purification of many different types of molecules. Its mobile phase also has a wide range, and different types of solvents can be used such as hexane, ethanol, and isopropanol. Additionally, there is no limit and any amount of mixture can be separated using this method. The process can also be automated and the separated analytes can be reused.

Additional resources

Separation techniques: Chromatography

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