What are the applications of column chromatography?

Its main application is to separate and purify single components from a mixture dissolved in fluid. Another application is that it is used for isolating active constituents. For example, It is used in the separation of plant extracts into its components in pure form. This technique is also used to estimate drugs in drug formulations or crude extracts. This makes it useful for pharmaceutical formulations. Column chromatography can also be used by toxicologists to isolate metabolites from biological fluids. This technique is also used to separate and purify both liquid and solid samples. Column chromatography is also used for forensic testing to analyze blood samples.