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AAT Bioquest

What are the advantages of restriction fragment length polymorphism (RELP) technique?

Posted June 4, 2024


Answer

Advantages of restriction fragment length polymorphism (RFLP):

  • No prior sequence information required 
  • No prior oligonucleotide synthesis required
  • Co-dominantly inherited and can thus estimate heterozygosity
  • Well suited for phylogenetic analysis between related species as it is based on sequence homology
  • Well suited for constructing genetic linkage maps
  • Results based on dependable genotypic characteristics rather than on phenotypes
  • Locus-specific markers allow synteny studies
  • Has discriminatory power, enabling single-locus probes at species and/or population levels or multi-locus probes at individual level
  • Highly robust methodology with good transferability between labs
Additional resources

An illustration of the advantages of efficient statistical methods for RFLP analysis in forensic science

DNA Fragmentation

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