What are the advantages of restriction fragment length polymorphism (RELP) technique?
Posted June 4, 2024
Answer
Advantages of restriction fragment length polymorphism (RFLP):
- No prior sequence information required
- No prior oligonucleotide synthesis required
- Co-dominantly inherited and can thus estimate heterozygosity
- Well suited for phylogenetic analysis between related species as it is based on sequence homology
- Well suited for constructing genetic linkage maps
- Results based on dependable genotypic characteristics rather than on phenotypes
- Locus-specific markers allow synteny studies
- Has discriminatory power, enabling single-locus probes at species and/or population levels or multi-locus probes at individual level
- Highly robust methodology with good transferability between labs
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