Advantages of restriction fragment length polymorphism (RFLP):

• No prior sequence information required 
• No prior oligonucleotide synthesis required
• Co-dominantly inherited and can thus estimate heterozygosity
• Well suited for phylogenetic analysis between related species as it is based on sequence homology
• Well suited for constructing genetic linkage maps
• Results based on dependable genotypic characteristics rather than on phenotypes
• Locus-specific markers allow synteny studies
• Has discriminatory power, enabling single-locus probes at species and/or population levels or multi-locus probes at individual level
• Highly robust methodology with good transferability between labs