What are the limitations of restriction fragment length polymorphism (RELP) technique?

Limitations of restriction fragment length polymorphism (RFLP): 

• Requires large amounts of sample DNA
• Can be slow and cumbersome
• No potential for automation
• Few loci detected per assay
• Low levels of polymorphism in some species requiring detection by radioisotope
• Single-copy probes can be time-consuming
• Requires a suitable probe library
• Requires the combined process of DNA fragmentation, probe labeling, electrophoresis, hybridization, washing, blotting and autoradiography